Ingénieur en bioinformatique et génomique

DescriptionInternship title: Integrative multi-Omic analysis for priming potential in a model forest and cultivated tree species: poplar.Keywords: Data analysis from Epigenomics and Transcriptomics, NGS data analysis (Long reads and WGBS), integrative statistical analysis and modelling.The P2e laboratory is conducting research into the epigenetics of trees in response to climate change, integrating ecophysiology, biochemistry, genetics and genomics. The ANR EPITREE project has explored the impact of this epigenetic mark on the adaptation of trees such as poplar. An M2 internship is proposed to focus on the integrative bioinformatics and statistical modelling of omics data obtained in collaboration with specialists in relation to a European network.A multi-omics analysis will be conducted, including MIXoMICS. Available data includes methylome (WGBS and sequence capture bisulfite), RNAseq data (long reads Oxford Nanopore), and bacteriome NGS data. An RNAseq analysis pipeline will be prepared according to Nanopore recommendations (gene expression, differential expression, RNA splicing). These candidate genes will be compared with the methylome data (pipeline already available) and several other analyses will be carried out, such as GO enrichment or gene network analyses, to extract biological interpretations in conjunction with the phenotyping data. Statistical analyses will be conducted, and high-quality graphs will be produced using R with a view to [email protected] #J-18808-Ljbffr